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Name: Leung Kin Man University number: 2009109720 Date of experiment: 25-2-2010 BIOL1133: biologic Science Laboratory Course Experiment 4: Expression of pieces secretin receptor concretion protein in E coli. Aim: The suggest of the experiment is to express human secretin receptor fusion protein in E coli by conducting SDS-PAGE gel dielectrolysis and estimate the molecular size of that protein. Abstract: In this experiment, the human secretin receptor fusion protein was expressed exploitation the SDS-PAGE technique. The pre-induced and the sample afterwards IPTG induction was use to extract the human secretin receptor protein by use denaturing buffer zone and elution buffer. The molecular weight of the protein bath also be estimated by comparing the bands on the protein ladder. accounting entry: Massive production of active protein is usually done by recombinant deoxyribonucleic acid technology. E coli. was employ as vector for interjection and exp ression of fusion protein. E coli. is a germ-negative bacteria with copy membrane layer and gamin plasmid. In this experiment, the N-terminal extracellular electron orbit of the human secretin receptor in E coli. was expressed using IPTG inducible system.

Secretin is the key hormone that prudent for the regulation of gastrointestinal knead by regulating the secretion of bicarbonate and enzyme from the exocrine pancreas. This good deal help to neutralize stomachic acid (hydrochloric acid) and hence harbour an optimum pH care for for intestinal digestion. The action of secretin is in general controlled by high-affinity membrane rece ptors distributed in our body and it is tiss! ue-specific. The N-terminal code realm of the extracellular domain of the receptor protein is obtained by conducting polymerase chain response (PCR) mutagenesis method. This technique was achieved by using specific deoxyribonucleic acid primers and DNA strand as a template to start the PCR reaction. The coding sequence was introduced into the bacterial DNA as a crew cut of the coding sequence. Upon IPTG...If you want to posture a full essay, purchase order it on our website: BestEssayCheap.com

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